Developer Naughty Dog has revealed that a patch to will bring PlayStation Trophy support to Uncharted: Drake's Fortune will be available by mid-August. It may hit sooner though as it has already been submitted for quality assurance.
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As with other PS3 titles getting retroactive Trophy support, Uncharted will require those who've already completed the game to play through again in order to unlock Trophies, even if they had previously earned every Medal. Wells says this is primarily to prevent people from simply using someone else's save data to artificially bolster their Trophy collection.